Will the root acces be available during this trial ?

Yes, root access can be available during the trial if you use a rooted image or manually enable it on a compatible unrooted image. For Android 10.0 and above, root is not enabled by default, but it can be toggled with the persist.sys.root_access setting in the UI, ADB, or API on supported products like Genymotion Desktop, SaaS, and PaaS: Root Access

On Android 9.0 and below, images are already rooted. On Android 10.0 and above, the web UI toggle is only available from Android 10.0 upward, while Android 11.0 and below note that the feature is not available on rooted Android images.